Effect of high-energy ionizing radiation on the DNA content and genetic variation in chrysanthemum plants regenerated from irradiated ovaries

This study aimed to evaluate the range of quantitative and qualitative genetic changes in chrysanthemum plants regenerated vitro from ovaries irradiated with high-energy photons (5, 10, 15 Gy) electrons (10 Gy). The highest DNA loss (up 11%) was observed originating treated 10 Gy high energy. AMOVA revealed significant differences between populations representing different mutagenic treatments (18% for RAPD-based 22% SCoT-based analysis). incidence strongly correlated applied type dose ionizing radiation. distances reference were photon (5.8% RAPD 1.7% SCoT) electron-treated explants (6.0% 2.9% SCoT). Considerable phenotype mutants not necessarily extent alterations. Qualitative methods evaluating post-radiation should be combined reliable detection variant at early developmental stages.